The object of study was to develop and compare the new method dose weight response curve with graphical dose response curve (DRC) method to study drug receptor interaction and dose response relationship in experimental pharmacology. The graphical DRC method was performed by adding graded doses of acetylcholine and it has produced dose dependent increase in tissue contraction and response. For dose weight response curve method the assembly was setup and same graded response of acetylcholine were added and it has produced dose dependent increase in tissue contraction and response in terms of weight reduction. For establishment and validation of dose weight response curve method different experiment were performed using isolated chicken ileum such as concentration response curve, potentiating effect and antagonistic effect and results compared with graphical dose response curve. The result suggests that a dose weight response curve method is simple, ease of setup of assembly, accurate, sensitive, precise and feasible for the purpose of experiment on isolated preparation and various bioassay methods.
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